| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Bacillus sonorensis | AGCCCTATTTCGAGCTGCG | AGCGACAACACATCGGCA | 60.23 | 59.97 | 160 | 124 |
100.00%
|
100.00%
|
| Primer 42 | Bacillus sonorensis | TGCTGGCCATGCAAGGTT | ATCAGCAAGCTCCATCCCG | 60.20 | 59.85 | 167 | 122 |
100.00%
|
100.00%
|
| Primer 43 | Bacillus sonorensis | TGCTGGCCATGCAAGGTT | AGCATGTCTCCCGTCGTT | 60.20 | 58.62 | 199 | 122 |
100.00%
|
100.00%
|
| Primer 44 | Bacillus sonorensis | TGCTGGCCATGCAAGGTT | AGAAGCATGTCTCCCGTCG | 60.20 | 59.49 | 202 | 122 |
100.00%
|
100.00%
|
| Primer 45 | Bacillus sonorensis | TGTCGGCAGCGTGACAAT | GCTTGTGATGCTTCGCCT | 59.97 | 58.42 | 242 | 122 |
100.00%
|
100.00%
|
| Primer 46 | Bacillus sonorensis | AAGCTTGAACGGCTGCCT | AAGCAGCCAGACAGCGAT | 59.89 | 59.33 | 204 | 122 |
100.00%
|
100.00%
|
| Primer 47 | Bacillus sonorensis | AAGCTTGAACGGCTGCCT | CCGCACCGACGAACAAAA | 59.89 | 58.98 | 220 | 122 |
100.00%
|
100.00%
|
| Primer 48 | Bacillus sonorensis | AAGCTTGAACGGCTGCCT | AAAAGCAGCCAGACAGCG | 59.89 | 58.65 | 206 | 122 |
100.00%
|
100.00%
|
| Primer 49 | Bacillus sonorensis | TCATCGCCATGGCAAGCA | TGACATGCTGTGCGTCCT | 60.05 | 59.26 | 210 | 122 |
100.00%
|
100.00%
|
| Primer 50 | Bacillus sonorensis | TCATCGCCATGGCAAGCA | TTGACATGCTGTGCGTCCT | 60.05 | 59.93 | 211 | 122 |
100.00%
|
100.00%
|